| 1 | Belleview Rattlers |
| 2 |  Bradford Tornadoes |
| 3 | Buchholz Bobcats |
| 4 | Clay County Blue Devils |
| 5 | Crescent City Raiders |
| 6 | Dunnellon Tigers |
| 7 | East Ridge Knights |
| 8 | Eastside Rams |
| 9 | Eustis Panthers |
| 10 | Fleming Island Golden Eagles |
| 11 |  Forest Wildcats |
| 12 | Gainesville Purple Hurricanes |
| 13 |  Hawthorne Hornets |
| 14 | Keystone Heights Indians |
| 15 | Lake Minneola Hawks |
| 16 |  Leesburg Yellow Jackets |
| 17 | Middleburg Broncos |
| 18 | Mount Dora Hurricanes |
| 19 | Newberry Panthers |
| 20 | North Marion Colts |
| 21 | Oakleaf Knights |
| 22 | Orange Park Raiders |
| 23 | P.K. Yonge Blue Wave |
| 24 | Palatka Panthers |
| 25 | Ridgeview Panthers |
| 26 | Santa Fe Raiders |
| 27 |  South Lake Eagles |
| 28 | St. Francis Catholic Wolves |
| 29 | Tavares Bulldogs |
| 30 |  Trinity Catholic Celtics |
| 31 | Umatilla Bulldogs |
| 32 | Vanguard Knights |
| 33 | West Port Wolf Pack |
